Details-enriched characterization of cell populations. Morphological information and facts from photographs can add towards the richness of flow cytometry facts. Such as, the figure shows an if not typical apoptosis assay employing Annexin V and PI, introducing cell imaging to characterize cells in Just about every population to expose morphologically unique features. These insights could not are attained from multiplex staining on your own.
As samples are acquired within the Attune CytPix Flow Cytometer, the higher-velocity brightfield digicam captures and suppliers photos of detected occasions at a charge around 6,000 images for every 2nd, according to the flow rate and graphic size. For bigger overall flexibility, Attune Cytometric Software permits you to alter the impression capture frequency as wanted and select a selected gate for image capture, if required.
(B) Uncooked 264.seven macrophages are frequently annotated inaccurately with respect to particle count because of their complexity and noncircular borders. The quantity of singlets readily available for Investigation improves as soon as the person trains and employs a far more precise product.
Find new insights into your experimental details by visualizing populations making use of put together fluorescence and morphology information. Our software program interface wizards allow downsampling, concatenation (merged data files) and dimensionality equipment which might be developed into the software for an intuitive, streamlined information analysis encounter.
"A picture isn't really value a thousand dots, but it surely really helps you to see photos. It is excellent to discover photos that correlate with staining. Such as, AnnexinV-beneficial cells which can be morphologically unique than healthier cells, or CD14+ monocytes that are more substantial and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
Below, a skilled person has gated singlets confidently. Soon after evaluating the handbook singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate contains a lot more than four% aggregates.
Pair your Attune flow cytometer with the autosampler for elevated workflow effectiveness, and increase robotic integration for maximum throughput.
Our experts will structure panels for yourself as again and again you may need—gratis, no product buy wanted.
Early signs of this contamination had originally been dismissed as particles. Backgating to find new populations. Backgating imaged cells on the Attune CytPix Flow Cytometer also enables you to use morphological capabilities to find out appealing subpopulations that would not be obvious from flow cytometry details by yourself.
Maybe most significantly, these functions incorporate cells of Plainly distinct phenotypes which could lead on to incorrect conclusions concerning double beneficial occasions (particularly in exceptional populations).
Seem waves empower specific mobile alignment, resulting in Remarkable facts high-quality and ~10X a lot quicker sample flow fees.
Application Be aware: Automatic picture Examination lessens person-to-user variability in flow cytometry gating approaches
Constructed-in dimensionality reduction equipment increased resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to confirm envisioned population resolution in lysed complete human blood. Consumer can watch occasions from Each individual cluster for visual confirmation of cell phenotypes.
Adaptable payment strategy from Agilent Financial Options assist you to get the crucial instrumentation you need for speedy task, or to develop your lab.
Gating on these extended parameters permits you to swiftly and correctly detect populations of curiosity and make sure gating technique with little or no manual review. The Attune computer software features usage of point out-of-the-artwork equipment Understanding analysis equipment inside an expertly made person interface, making it exceptionally convenient to use.
As the cell line had Formerly been analyzed about the Attune CytPix Flow Cytometer, the researchers went again to the photographs and were in the position to here document the microbial infection no less than five times previously.
The Attune CytPix Flow Cytometer enables end users to visualize samples throughout acquisition, validate the precision of gating tactics, and guidance effects with trusted documentation for publication functions.
In this article, we display that by gating procedures working with picture-centered quantitative parameters can distinguish interacting cells from coincident situations to a lot more precisely evaluate interacting cells.